# ############################################## # Please fill these blanks after the colon. # ############################################## # Make sure all space are replaced by dot(.). For example, # Average Intensity Ratio is replaced by Average.Intensity.Ratio. # # The location of the files generated from the Columbus system datapath : # The location of output files outpath : # The control well ID. # Leave case.wells as blank will include all wells except positive & negative # control and neglect.well. Please write in one line seperated by comma (,). positive.control : negative.control : neglect.well : case.well : # The location of the well-gene specification file (.csv (comma separator)). # Make sure the first row is the colum names, eg. Barcode, Well, GeneSymbol genemap : # The organism organism : # The location of the file name specification file (.csv (comma separator)). # Leave it blank if you have a consistent report format and file name format. platemap : # The format of the files, Matrix or Tab (Table format) cellformat : # # ########################################### # Please check these paramters # ########################################### # # An example of the file name. For example, if your file names # for.example_0205-s2-1.txt means the experiment is performed on plate 1, # and it is the second replicate, the barcode showed on the well-gene # specification file is DSIHGA01, the paramters are as follows. eg.filename : for.example_0205-s2-1.txt rep.id : s2 exp.id : 1 sep : - barcode : DSIHGA01 # In the well-gene specification file, if the well ID is B1, B2, ..., B11, # the well.digits = 1; while B01, B02, ..., B11, the well.digits = 2; # and B001, B002, ..., B011, the well.digits = 3. well.digits : 2 # Analyze items. For example: Average.Intensity.of.Nuclei. # We add a new item Average.Total.Intensity if you are interested in the total # intensity from both the nucleus and the cytoplasm. # Please write in one line seperated by comma (,). cellnames : Average.Intensity.of.Nuclei, Average.Intensity.of.Cytoplasm, Average.Total.Intensity, Average.Intensity.Ratio # Normalization method MP, PMed, Z, Ctr, None norm.method : MP # Quality control method plateCorrelation, wellSd, zFactor, cellNumber opm.qcType : plateCorrelation, wellSd, zFactor, cellNumber # Quality control threshold correlationTh : 0.8 zfactorTh : 0.5 cellnumberTh : 50 if.replace.badPlateData : TRUE # Hit detection method stable, ksd, kmsd sig.method : stable sig.threshold : 0.05 sig.pvalue.threshold : 0.05 # Require a detailed report or only the selected hit list verbose : TRUE # The report file name summaryFile : summary.txt # The report of gene enrichment analysis functionFile : functions.txt